TARDBP antibody (C-Term, Cleavage Site)

Catalog No. ABIN784609

This Rabbit Polyclonal antibody specifically detects TARDBP in WB and IHC (fro). It exhibits reactivity toward Human and Rat.

Quick Overview for TARDBP antibody (C-Term, Cleavage Site) (ABIN784609)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TARDBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TARDBP Antibody

Binding Specificity: C-Term, Cleavage Site

Specificity: Specific for the ~ 43 kDa TDP-43 protein in Western blots of Rat brain lysate. Expected molecular weight is dependent upon the TDP-43 species present in sample (full-length vs. truncated TDP-43). Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in Human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43. Under denaturing conditions (for example, by Western Blot analysis) this antibody detects the C-terminus of full-length and truncated Human TDP-43.

Cross-Reactivity (Details): Species reactivity (tested):Human and Rat.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide C-terminal to the caspase-cleavage site (between D219 and V220) of Human TDP-43.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 30 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -80 °C.

Target Details for TARDBP

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: TARDBP

Background: TDP43 (Tar DNA Binding 43, TARDBP) was originally identified as a protein which binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (Ou et al.,1995). UV cross-linking of HeLa cell extract revealed a 43 kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (Neumann et al., 2006). The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms.Synonyms: TAR DNA-binding protein 43, TDP-43, TDP43

Gene ID: 23435

NCBI Accession: NP_031401

Pathways: Positive Regulation of Peptide Hormone Secretion