GCK antibody (N-Term)

Catalog No. ABIN786429

Cited in 1 publication. The Rabbit Polyclonal anti-GCK antibody (ABIN786429) specifically detects GCK in WB. The antibody is reactive with Human, Rat, Mouse, Cow, Guinea Pig, Rabbit, Sheep, Dog and Horse samples.

Quick Overview for GCK antibody (N-Term) (ABIN786429)

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reactivity: Human, Rat, Mouse, Cow, Guinea Pig, Rabbit, Sheep, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GCK Antibody

Binding Specificity: N-Term

Purpose: GCK Antibody - N-terminal region

Sequence: RVMLVKVGEG EEGQWSVKTK HQMYSIPEDA MTGTAEMLFD YISECISDFL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against GCK. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP61473-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GCK antibody (ABIN786429)

Tian, Liu, Wang, Dong, Su, Ma, Zhang: "Anti-diabetes effect of chronic intermittent hypobaric hypoxia through improving liver insulin resistance in diabetic rats." in: Life sciences, Vol. 150, pp. 1-7, (2016) (PubMed).

Target Details for GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: GCK

Background:

Background Information: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Gene Name: Glucokinase (hexokinase 4)

Alternative Symbols: GK, GLK, HK4, HHF3, HKIV, HXKP, LGLK, MODY2, PNDM1, FGQTL3

Protein Name: Glucokinase

Molecular Weight: 51kDa

Gene ID: 2645

NCBI Accession: NP_277043

UniProt: P35557

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process