Small Basement Membrane Heparan Sulfate Proteoglycan antibody
Reactivity: Human
WB, IHC, ELISA
Host: Mouse
Monoclonal
1F10-B8
unconjugated
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- Target
- Small Basement Membrane Heparan Sulfate Proteoglycan
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Specificity
- Monoclonal Ab 1F10/B8 is specific for a core protein epitope of a human small basement membrane heparan sulfate proteoglycan (HS-PG). MAb 1F10/B8 recognizes an epitope different from that recognized by mAb 2H7/G11 and mAb 2E2/B5. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that at least two other basement membrane HS-PG exist: Agrin, originally discovered as an important component of the neuromuscular junction, and a novel small HS-PG which was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohistochemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.
- Purification
- Purified human small basement membrane heparan sulfate proteoglycan (HS-PG). Protein G purified.
- Clone
- 1F10-B8
- Isotype
- IgG1 kappa
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- Application Notes
- ELISA (less than 1µg/ml), Western Blot (1µg/ml), immunohistochemistry (1µg/ml). This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Resuspend in aqua bidest.
- Buffer
- 50 mM TRIS pH 7.4
- Storage
- 4 °C
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- Target
- Small Basement Membrane Heparan Sulfate Proteoglycan
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