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Nanog Homeobox (NANOG) (AA 137-141) antibody

Details for Product No. ABIN871478, Supplier: Log in to see
Antigen
Epitope
AA 137-141
26
21
19
17
14
14
13
13
13
12
9
8
8
6
5
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
281
61
34
6
4
2
1
Host
Rabbit
208
62
37
5
Clonality
Polyclonal
Conjugate
Un-conjugated
14
14
14
12
12
12
3
2
2
2
2
2
2
2
2
1
Application
Western Blotting (WB)
226
159
83
66
37
37
30
26
22
14
6
4
3
3
2
2
1
1
1
1
1
Supplier
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Immunogen Peptide sequence around AA 137-141 (K-Q-V-K-T) derived from Nano g Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Specificity The antibody detects endogenous level of total Nanog protein.
Purification Affinity purified
Alternative Name Nanog (NANOG Antibody Abstract)
Background Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation
Molecular Weight 42 kDa
Gene ID 79923
NCBI Accession NP_079141
UniProt Q9H9S0
Research Area Embryogenesis, Embryonic stem cells, Cell Cycle
Pathways
Application Notes Western blotting: 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Freberg, Dahl, Timoskainen et al.: "Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract." in: Molecular biology of the cell, Vol. 18, Issue 5, pp. 1543-53, 2007 (PubMed).

Do, Lim, Kim et al.: "An intact homeobox domain is required for complete nuclear localization of human Nanog." in: Biochemical and biophysical research communications, Vol. 353, Issue 3, pp. 770-5, 2007 (PubMed).

Boyer, Lee, Cole et al.: "Core transcriptional regulatory circuitry in human embryonic stem cells." in: Cell, Vol. 122, Issue 6, pp. 947-56, 2005 (PubMed).

Chambers, Colby, Robertson et al.: "Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells." in: Cell, Vol. 113, Issue 5, pp. 643-55, 2003 (PubMed).