Nanog antibody (AA 137-141)
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- Target See all Nanog (NANOG) Antibodies
- Nanog (NANOG) (Nanog Homeobox (NANOG))
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Binding Specificity
- AA 137-141
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Nanog antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- The antibody detects endogenous level of total Nanog protein.
- Purification
- Affinity purified
- Immunogen
- Peptide sequence around AA 137-141 (K-Q-V-K-T) derived from Nano g Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
- Top Product
- Discover our top product NANOG Primary Antibody
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- Application Notes
- Western blotting: 1:1000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- Target
- Nanog (NANOG) (Nanog Homeobox (NANOG))
- Alternative Name
- Nanog (NANOG Products)
- Synonyms
- 2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody
- Background
- Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation
- Molecular Weight
- 42 kDa
- Gene ID
- 79923
- NCBI Accession
- NP_079141
- UniProt
- Q9H9S0
- Pathways
- Stem Cell Maintenance
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