Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 311-315) antibody

Details for Product No. ABIN871525
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Antigen
Synonyms MEK5, MAPKK5, PRKMK5, HsT17454, Mapkk5, Prkmk5, AI324775, AI428457, Mek5, fb73f02, wu:fb73f02, zgc:172137
Epitope
AA 311-315
(25), (24), (12), (12), (12), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus), Mouse (Murine)
(88), (64), (56), (54), (41), (40), (24), (4), (3), (2), (1), (1)
Host
Rabbit
(96), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Western Blotting (WB)
(53), (50), (25), (17), (6), (4), (3)
Pubmed 4 references available
Catalog no. ABIN871525
Quantity 50 µL
Price
165.00 $   Plus shipping costs $45.00
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Immunogen Peptide sequence around AA 311-315 (K-T-Y-V-G ) derived from Rat MEK5. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Specificity The antibody detects endogenous level of total MEK5 protein.
Purification Affinity purified
Alternative Name MEK5
Background A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
Molecular Weight 49 kDa
Gene ID 29568
NCBI Accession NP_001029
UniProt Q62862
Research Area Cancer, Kinases/Phosphatases
Application Notes Western blotting: 1:500
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Han, Lee, Jiang et al.: "Characterization of the structure and function of a novel MAP kinase kinase (MKK6)." in: The Journal of biological chemistry, Vol. 271, Issue 6, pp. 2886-91, 1996 (PubMed).

Jiang, Chen, Li et al.: "Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta)." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17920-6, 1996 (PubMed).

Tournier, Whitmarsh, Cavanagh et al.: "Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 14, pp. 7337-42, 1997 (PubMed).

Holland, Suzanne, Campbell et al.: "MKK7 is a stress-activated mitogen-activated protein kinase kinase functionally related to hemipterous." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 24994-8, 1997 (PubMed).

Hosts (96), (3)
Reactivities (88), (64), (56), (54), (41), (40), (24), (4), (3), (2), (1), (1)
Applications (53), (50), (25), (17), (6), (4), (3)
Conjugates (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (25), (24), (12), (12), (12), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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