CHEK1 antibody

Catalog No. ABIN3209997

The Mouse Monoclonal anti-CHEK1 antibody (Clone 2G1D5) (ABIN3209997) specifically detects CHEK1 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for CHEK1 antibody (ABIN3209997)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2G1D5

Product Details anti-CHEK1 Antibody

Cross-Reactivity: Mouse (Murine)

Purification: Purified antibody.

Immunogen: CHK1 antibody was raised in Mouse using a purified recombinant fragment of CHK1 expressed in E. coli as the immunogen.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10,000, WB: 1:500-1:2,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Purified antibody supplied in PBS containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Store at -20 °C for long term storage.

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1

Background: CHK1 is a kinase that phosphorylates Cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Synonyms: Monoclonal CHK1 antibody, Anti-CHK1 antibody, CHK-1 antibody, CHK 1 antibody, CHEK1 antibody.

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair