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Metabotropic Glutamate Receptor 1 antibody (Glutamate Receptor, Metabotropic 1)

Details for Product anti-GRM1 Antibody No. ABIN93988, Supplier: Log in to see
Antigen
  • fj39d05
  • wu:fj39d05
  • si:ch211-51a19.1
  • Glura
  • mGlutR
  • GB18621
  • GPRC1A
  • MGLU1
  • MGLUR1
  • SCAR13
  • 4930455H15Rik
  • ENSMUSG00000075319
  • Gm10828
  • Gprc1a
  • mGluR1
  • nmf373
  • rcw
  • wobl
Reactivity
Human, Non-Human Primate
119
41
34
6
3
3
1
Host
Mouse
112
16
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Metabotropic Glutamate Receptor 1 antibody is un-conjugated
5
5
5
5
4
4
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
83
52
52
29
12
9
9
3
3
2
2
2
2
1
1
Supplier
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Immunogen Normal human peripheral blood granulocytes
Clone LNK16
Isotype IgG1
Specificity The antibody LNK16 reacts with CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils).HLDA V, WS Code M MA069HLDA V, WS Code NK NK50
Purification Purified from ascites by precipitation methods and ion exchange chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name GRM1 (GRM1 Antibody Abstract)
Background CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms -, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Pathways
Application Notes Flow Cytometry: Recommended dilution: 2 µg/mL

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-Metabotropic Glutamate Receptor 1 antibody (Glutamate Receptor, Metabotropic 1) (ABIN93988) Surface staining of human peripheral blood cells with anti-CD16 (LNK16) PE.
Product cited in: Hovden, Karlsen, Jonsson et al.: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011 (PubMed).

Boyle: "Human macrophages kill human mesangial cells by Fas-L-induced apoptosis when triggered by antibody via CD16." in: Clinical and experimental immunology, Vol. 137, Issue 3, pp. 529-37, 2004 (PubMed).

Mathison, Befus, Davison et al.: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Tamm, Schmidt: "The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 4, pp. 1576-81, 1996 (PubMed).

Background publications Mehrle, Watzl, von Lilienfeld-Toal et al.: "Comparison of phenotype of gammadelta T cells generated using various cultivation methods." in: Immunology letters, Vol. 125, Issue 1, pp. 53-8, 2009 (PubMed).

Meknache, Joensson, Laurent et al.: "Human basophils express the glycosylphosphatidylinositol-anchored low-affinity IgG receptor FcgammaRIIIB (CD16B)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 4, pp. 2542-50, 2009 (PubMed).

Kahn, Moergelin, Shannon et al.: "Antibodies against a surface protein of Streptococcus pyogenes promote a pathological inflammatory response." in: PLoS pathogens, Vol. 4, Issue 9, pp. e1000149, 2008 (PubMed).

Chen, Freedman: "CD16+ gammadelta T cells mediate antibody dependent cellular cytotoxicity: potential mechanism in the pathogenesis of multiple sclerosis." in: Clinical immunology (Orlando, Fla.), Vol. 128, Issue 2, pp. 219-27, 2008 (PubMed).

Mathison, Christie, Davison: "The tripeptide feG inhibits leukocyte adhesion." in: Journal of inflammation (London, England), Vol. 5, pp. 6, 2008 (PubMed).

Congy-Jolivet, Bolzec, Ternant et al.: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." in: Cancer research, Vol. 68, Issue 4, pp. 976-80, 2008 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Arase, Arase, Hirano et al.: "IgE-mediated activation of NK cells through Fc gamma RIII." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 6, pp. 3054-8, 2003 (PubMed).

Kocher, Siegel, Edberg et al.: "Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 8, pp. 3940-8, 1997 (PubMed).

Gessner, Grussenmeyer, Kolanus et al.: "The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions." in: The Journal of biological chemistry, Vol. 270, Issue 3, pp. 1350-61, 1995 (PubMed).