CD2 antibody (CD2 Molecule)

Details for Product anti-CD2 Antibody No. ABIN94021, Supplier: Log in to see
Antigen
  • CD2
  • LFA-2
  • Ly-37
  • Ly37
  • SRBC
  • T11
  • CD2R
  • LFA2
  • OX-34
  • OX34
  • CD2 molecule
  • CD2 antigen
  • Cd2 molecule
  • CD2
  • Cd2
Reactivity
Human
590
149
124
34
27
25
19
16
15
12
11
10
7
5
4
3
3
2
1
Host
Mouse
634
116
94
4
Clonality (Clone)
Monoclonal ()
Conjugate
This CD2 antibody is un-conjugated
83
79
69
20
20
19
17
17
17
14
13
13
13
13
13
11
9
9
5
5
5
5
5
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
656
175
173
139
126
111
107
103
92
46
13
12
10
9
8
7
4
4
4
3
2
2
2
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Human peripheral T cells.
Clone MEM-65
Isotype IgG1
Specificity The antibody MEM-65 recognizes an unique epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T-lymphocytes and NK cells, also expressed by all thymocytes.
HLDA VI, WS Code T 6T-012
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD2 (CD2 Antibody Abstract)
Background CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD2 antibody (CD2 Molecule) (ABIN94021) Surface staining of human peripheral blood with anti-human CD2 (MEM-65) purified, GAM...
Background publications Muhammad, Schiller, Forster, Eckerstorfer, Geyeregger, Leksa, Zlabinger, Sibilia, Sonnleitner, Paster, Stockinger: "Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7672-80, 2009 (PubMed).

Zhu, Dustin, Cairo, Thatte, Golan: "Mechanisms of Cellular Avidity Regulation in CD2-CD58-Mediated T Cell Adhesion." in: ACS chemical biology, Vol. 1, Issue 10, pp. 649-58, 2006 (PubMed).

Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).

Product cited in: Espagnolle, Depoil, Zaru, Demeur, Champagne, Guiraud, Valitutti: "CD2 and TCR synergize for the activation of phospholipase Cgamma1/calcium pathway at the immunological synapse." in: International immunology, Vol. 19, Issue 3, pp. 239-48, 2007 (PubMed).

Drbal, Hilgert, Cebecauer, Angelisová, Horejsí: "New monoclonal antibodies to human leucocyte surface molecule CD2." in: Folia biologica, Vol. 43, Issue 6, pp. 245-6, 1998 (PubMed).