phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) antibody

Antigen: phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1)

Synonyms: CBP, RSTS, KAT3A, PAG, FLJ37858, MGC138364, Cbp, Pag, F730007C19Rik, MGC80192

Clonality: Monoclonal (MEM-255)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94272

Additional Information

Alternative name: PAG / Cbp

Immunogen: Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: MEM-255

Description: PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is aubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts(glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gelsanomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src familykinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the majornegative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells orhigh affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosinephosphorylation and Csk binding, while T cell receptor signaling causes PAGdephosphorylation, loss of Csk binding and increased activation of the protein tyrosinekinase Lck.

Specificity: The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp)located in the cytoplasmic domain, also known as protein associated withglycosphingolipid-enriched microdomains (PAG). This antibody does not react with: Mouse, Rat, Bovine.

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 2 µg/ml. Application note: intracellular staining, permeabilization requiredWestern Blotting. Application note: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor proteinpresent in membrane microdomains (rafts), which however migrates on SDS-PAGE gelsanomalously as an 80 kDa molecule. Immunohistochemistry (paraffin sections) - Positive tissue: tonsil, spleen

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunohistochemistry of paraffin-embedded sections

Publications

Publications: Yerly, Ding, Tauzin et al.: "The sphingolipid-rich rafts of ALK+ lymphomas downregulate the Lyn-Cbp/PAG signalosome." in: European journal of haematology, 2010 (PubMed).