Cytokeratin 5/18 antibody

Antigen: Cytokeratin 5/18

Clonality: Monoclonal (C-50)

Host: Mouse

Reactivity: Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Hamster, Cow (Bovine), Sheep (Ovine), Dog (Canine)

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN94292

Additional Information

Alternative name: Cytokeratin 5+18

Immunogen: Cytoskeleton preparation from HeLa cells.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: C-50

Description: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Specificity: The antibody C-50 reacts with Cytokeratin peptides 5 and 18 (58 and 45 kDa). Cytokeratinsare a member of intermediate filaments subfamily represented in epithelial tissues. This antibody does not react with: Chicken, Xenopus, Other not tested.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Images

Western Blotting