Mouse anti-Human IgG (Fab Region) Antibody

Details for Product No. ABIN94400, Supplier: Log in to see
Antigen
Epitope
Fab Region
4684
2573
1882
1039
1015
825
305
272
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
2780
2759
2347
1724
1436
778
607
426
364
338
330
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
Host
Mouse
6750
4661
1834
811
684
394
88
52
51
9
8
5
5
4
2
1
1
Clonality (Clone)
Monoclonal ()
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
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Immunogen Purified human IgG.
Clone 4A11
Isotype IgG2b
Specificity The antibody 4A11 reacts with Fab fragment of human IgG.
Purification Purified from ascites by precipitation methods and ion exchange chromatography.
Purity > 95 % (by SDS-PAGE)
Background Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Molecular Weight 150 kDa
Research Area Immunology, Secondary Antibodies
Application Notes Working concentrations should be determined by the investigator.I t is recommended that the user titrates the reagent for use in the particular testing system.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for Mouse anti-Human IgG (Fab Region) Antibody (ABIN94400) Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).