Mouse anti-Cow (Bovine) IgG (Light Chain) Antibody

Details for Product No. ABIN94401, Supplier: Log in to see
Antigen
Epitope
Light Chain
4684
2573
1882
1039
1015
824
305
273
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine)
2780
2760
2347
1724
1436
778
607
425
364
338
330
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
Host
Mouse
6750
4661
1834
811
684
394
88
52
51
9
8
5
5
4
2
1
1
Clonality (Clone)
Monoclonal ()
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Immunogen Fraction of Bovine immunoglobulin.
Clone IVA285-1
Isotype IgG1
Specificity The antibody IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nad lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Research Area Immunology, Secondary Antibodies
Application Notes Western Blotting: Recommended dilution: 0,5-1 µg/mL, overnight in 4 °C
Positive control and sample preparation: BS (bovine serum), dilution 1: 50 in Laemmli reducing buffer, boiled in water bath for 3 min
Application notes: Strongly reacts with bovine IgG light chains, weakly reacts with IgM.Reducing conditions. SDS-PAGE (12 % separating gel).
Flow Cytometry:

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for Mouse anti-Cow (Bovine) IgG (Light Chain) Antibody (ABIN94401) Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and B...
Product cited in: Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, 2006 (PubMed).