P21/WAF1 antibody

Antigen: P21/WAF1

Clonality: Monoclonal (WA-1)

Host: Mouse

Reactivity: Human, Primates

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunocytochemistry (ICC)

Catalog no.: ABIN94444

Additional Information

Alternative name: p21Waf1

Immunogen: Human p21 protein

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: WA-1

Description: The tumour suppressor p21Waf1 (Wild-type p53-Activated Fragment 1, also known as Cip1,Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which isexpressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors uponvarious stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibitsCdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA(proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21counteracts its inhibitory activities.

Specificity: The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1), a 21 kDa tumoursuppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferatingcell nuclear antigen.

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - Positive tissue: colon carcinomaImmunocytochemistryELISA

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunohistochemistry staining of colon carcinoma (paraffin-embedded sections) with anti-p21 (WA-1).