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Tumor Protein P53 (TP53) antibody
| Antigen | Tumor Protein P53 (TP53) |
| Synonyms | p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53 |
| Clonality | Monoclonal (BP53-12) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human, Primates |
| Application |
Alternatives Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunocytochemistry (ICC)
|
| Catalog no. | ABIN94448 |
| Quantity | 0.1 mg (1 mg/ml) (Variants) |
| Price | 273.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 3 to 5 Business Days |
Additional Information
| Alternative name | p53 |
| Immunogen | Bacterially expressed full-length wild-type p53 |
| Format | Purified |
| Isotype | IgG2a (Matching secondary antibodies) |
| Clone | BP53-12 |
| Description | The tumour suppressor protein p53 is a key element of intracellular anticancer protection. Itmediates cell cycle arrest or apoptosis in response to DNA damage or to starvation forpyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulatedto activate transcription of specific genes, resulting in expression of p21waf1 and otherproteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. Thestructure of p53 comprises N-terminal transactivation domain, central DNA-binding domain,oligomerisation domain, and C-terminal regulatory domain. There are variousphosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53activation and stabilization. |
| Specificity | The antibody BP53-12 recognizes defined epitope (aa 16-25) on human p53, a 50 kDatumour suppressor found in increased amounts in a wide variety of transformed cells, it isfrequently mutated or inactivated in many types of cancer. |
Application Details
| Application Notes | Western Blotting - Recommended dilution: 1-2 µg/ml, overnight in 4 o C Positive control: RAMOS human lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12% separating gel). |
| Concentration | 1 mg/ml |
| Purity | > 95% (by SDS-PAGE) |
| Purification | Purified from ascites by precipitation methods. |
| Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage | Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles. |
| Research Area | Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis |
| Restrictions | For Research Use only |
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Alternatives
Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
