Tumor Protein P53 (TP53) antibody (FITC)

Antigen: Tumor Protein P53 (TP53)

Synonyms: p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53

Clonality: Monoclonal (BP53-12)

Host: Mouse

Reactivity: Human, Primates

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN94449

Additional Information

Alternative name: p53

Immunogen: Bacterially expressed full-length wild-type p53

Isotype: IgG2a

Clone: BP53-12

Description: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. Itmediates cell cycle arrest or apoptosis in response to DNA damage or to starvation forpyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulatedto activate transcription of specific genes, resulting in expression of p21waf1 and otherproteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. Thestructure of p53 comprises N-terminal transactivation domain, central DNA-binding domain,oligomerisation domain, and C-terminal regulatory domain. There are variousphosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53activation and stabilization.

Specificity: The antibody BP53-12 recognizes defined epitope (aa 16-25) on human p53, a 50 kDatumour suppressor found in increased amounts in a wide variety of transformed cells, it isfrequently mutated or inactivated in many types of cancer.

Application Details

Application Notes: The reagent is designed for direct immunofluorescence analysis. Note: This conjugate is routinely tested by flow cytometry analysis using permeabilizedtransient wild-type p53-tranfectants.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Confocal microscopy of human HeLa cells using anti-p53 (BP53-12, FITC). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC MAb.Photo provided by Dr. Hodny, Inst. of Experimental Medicine, Prague, Czech Republic