Tumor Protein P53 (TP53) (pSer392) antibody

Antigen: Tumor Protein P53 (TP53)

Synonyms: p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53

Epitope: pSer392

Clonality: Monoclonal (FP3-2 [FPS392])

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94450

Additional Information

Alternative name: p53 (Phospho-Ser392)

Immunogen: KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids378-393 of human p53.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: FP3-2 [FPS392]

Description: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. Itmediates cell cycle arrest or apoptosis in response to DNA damage or to starvation forpyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulatedto activate transcription of specific genes, resulting in expression of p21waf1 and otherproteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. Thestructure of p53 comprises N-terminal transactivation domain, central DNA-binding domain,oligomerisation domain, and C-terminal regulatory domain. There are variousphosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53activation and stabilization.

Specificity: The antibody FP3.2 [FPS392] reacts with human p53 tumour suppressor proteinphosphorylated at CKII site (Ser 392).

Application Details

Application Notes: Western Blotting - Recommended dilution: 1 µg/ml. Immunohistochemistry (paraffin sections) - Staining technique: standard ABC technique (DAB+)Pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mMcitrate buffer pH 6. 0 or 1 mM EDTA-NaOH buffer pH 8. 0Recommended dilution: 10 µg/ml Incubation: 1 hour at RT, or overnight at 4 o C Positive tissue: breast carcinoma with high level of wild-type p53

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraffin-embedded sections).1A - anti-p53 (total)1B - anti-p53 (phospho Ser392)Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.

Publications

Publications: Blaydes, Craig, Wallace et al.: "Synergistic activation of p53-dependent transcription by two cooperating damage recognition pathways." in: Oncogene, Vol. 19, Issue 34, pp. 3829-39, 2000 (PubMed).