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Phosphotyrosine antibody

Details for Product No. ABIN94452, Supplier: Log in to see
Antigen
Reactivity
Broad Bean (Vicia faba)
107
51
46
29
28
27
25
9
9
2
1
Host
Mouse
175
63
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
27
24
17
14
7
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
176
110
85
79
73
53
27
25
11
7
7
2
1
1
1
1
1
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Immunogen Phosphotyrosine conjugated to bovine serum albumin.
Clone P-Tyr-01
Isotype IgG1
Specificity The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Immunofluorescence (IF) image for anti-Phosphotyrosine antibody (ABIN94452) Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody-Alexa Fluor &#...
Product cited in: Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, 2007 (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Background publications Brdi?ka, Cerný, Horej?í: "T cell receptor signalling results in rapid tyrosine phosphorylation of the linker protein LAT present in detergent-resistant membrane microdomains." in: Biochemical and biophysical research communications, Vol. 248, Issue 2, pp. 356-60, 1998 (PubMed).

Cebecauer, Cerný, Horejsí: "Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells." in: Biochemical and biophysical research communications, Vol. 243, Issue 3, pp. 706-10, 1998 (PubMed).