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Phosphotyrosine antibody

Details for Product No. ABIN94452, Supplier: Log in to see
Antigen
Reactivity
Amino Acid
109
52
43
29
29
27
25
9
9
5
1
Host
Mouse
178
63
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
27
24
17
15
7
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
177
110
85
77
72
50
28
27
11
7
7
2
1
1
1
1
1
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Immunogen pTyr conjugated to bovine serum albumin.
Clone P-Tyr-01
Isotype IgG1
Specificity The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Target Type Amino Acid
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Pathways
Application Notes Western Blotting: Recommended dilution: 1-2 µg/mL

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Immunofluorescence (IF) image for anti-Phosphotyrosine antibody (ABIN94452) Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody-Alexa Fluor &#...
Product cited in: Meraner, Horejsí, Wolpl et al.: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, 2007 (PubMed).

Brdicka, Imrich, Angelisová et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Background publications Brdi?ka, Cerný, Horej?í: "T cell receptor signalling results in rapid tyrosine phosphorylation of the linker protein LAT present in detergent-resistant membrane microdomains." in: Biochemical and biophysical research communications, Vol. 248, Issue 2, pp. 356-60, 1998 (PubMed).

Cebecauer, Cerný, Horejsí: "Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells." in: Biochemical and biophysical research communications, Vol. 243, Issue 3, pp. 706-10, 1998 (PubMed).