STAT-Induced STAT Inhibitor 3 (SOCS3) antibody

Antigen: STAT-Induced STAT Inhibitor 3 (SOCS3)

Synonyms: CIS3, SSI3, ATOD4, Cish3, SSI-3, SOCS-3, MGC71791, Cis3, Ef10, Ssi3, EF-10

Clonality: Monoclonal (SO1)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN94467

Additional Information

Alternative name: SOCS3

Immunogen: Full-length SOCS3 protein.

Format: Purified

Isotype: IgG2b  (Matching secondary antibodies)

Clone: SO1

Description: SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 isinduced by a variety of cytokines and other stimuli, such as erythropoietin, leptin andlipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNKphosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferativeresponses and is involved also in maintaining leukocytes in quiescent state until antigenstimulation.

Specificity: The antibody SO1 reacts with SOCS3, an intracellular cytokine signaling inhibitor.

Application Details

Application Notes: Western Blotting - Positive control: HeLa human cervix carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withreducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cancer, Hematopoietic Progenitors, Innate Immunity, Cytokines, Signaling

Restrictions: For Research Use only

Images

Western Blotting