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Afamin antibody (AFM) (N-Term)

Details for Product anti-AFM Antibody No. ABIN950291, Supplier: Log in to see
Antigen
  • AFM
  • ALB2
  • ALBA
  • ALF
  • Alf
  • alpha-Alb
Epitope
N-Term, AA 55-85
5
5
5
4
3
2
2
2
2
2
1
1
Reactivity
Human
50
22
16
3
3
1
1
1
Host
Rabbit
41
12
3
1
Clonality
Polyclonal
Conjugate
This Afamin antibody is un-conjugated
5
4
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
40
23
11
6
3
3
2
1
1
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 55~85 amino acids from the N-terminal region of human AFM
Isotype Ig
Specificity This antibody reacts to Afamin.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Alternative Name Afamin / AFM (AFM Antibody Abstract)
Background AFM is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.Synonyms: ALB2, ALBA, Alpha-albumin
Gene ID 173
NCBI Accession NP_001124
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Afamin antibody (AFM) (N-Term) (ABIN950291) Western blot analysis of AFM Antibody (N-term) in HepG2 cell line lysates (35µg/lane)...
Background publications Dieplinger, Ankerst, Burges et al.: "Afamin and apolipoprotein A-IV: novel protein markers for ovarian cancer." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 18, Issue 4, pp. 1127-33, 2009 (PubMed).

Kratzer, Bernhart, Wintersperger et al.: "Afamin is synthesized by cerebrovascular endothelial cells and mediates alpha-tocopherol transport across an in vitro model of the blood-brain barrier." in: Journal of neurochemistry, Vol. 108, Issue 3, pp. 707-18, 2009 (PubMed).

Ramachandran, Boontheung, Xie et al.: "Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." in: Journal of proteome research, Vol. 5, Issue 6, pp. 1493-503, 2006 (PubMed).

Liu, Qian, Gritsenko et al.: "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 6, pp. 2070-80, 2005 (PubMed).

Hu, Malone, Fagan et al.: "Comparative proteomic analysis of intra- and interindividual variation in human cerebrospinal fluid." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 12, pp. 2000-9, 2005 (PubMed).

Bunkenborg, Pilch, Podtelejnikov et al.: "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry." in: Proteomics, Vol. 4, Issue 2, pp. 454-65, 2004 (PubMed).