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AFF4 antibody (AF4/FMR2 Family, Member 4) (N-Term)

Details for Product anti-AFF4 Antibody No. ABIN950293, Supplier: Log in to see
Antigen
  • AFF4
  • RA_m002_jsmFBA6Br
  • AF5Q31
  • MCEF
  • Alf4
  • Laf4l
Epitope
N-Term, AA 1-30
18
17
4
2
2
2
2
2
1
1
1
Reactivity
Human
58
22
20
Host
Rabbit
51
8
Clonality
Polyclonal
Conjugate
This AFF4 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
41
24
11
4
4
2
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human AF5q31
Isotype Ig
Specificity This antibody reacts to AFF4.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography on Protein A
Alternative Name AFF4 / MCEF (AFF4 Antibody Abstract)
Background Synonyms: AF4/FMR2 family member 4, AF5Q31, ALL1-fused gene from chromosome 5q31, HSPC092, Major CDK9 elongation factor-associated protein
Gene ID 27125
NCBI Accession NP_055238
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-AFF4 antibody (AF4/FMR2 Family, Member 4) (N-Term) (ABIN950293) Western blot analysis of AF5q31 Antibody (N-term) in HL-60 cell line lysates (35µg/la...
Background publications Lin, Smith, Takahashi et al.: "AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia." in: Molecular cell, Vol. 37, Issue 3, pp. 429-37, 2010 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Niedzielski, Hopewell, Ismail et al.: "MCEF is localized to the nucleus by protein sequences encoded within three distinct exons, where it represses HIV-1 Tat-transactivation of LTR-directed transcription." in: International journal of biological sciences, Vol. 3, Issue 4, pp. 225-36, 2007 (PubMed).

Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Estable, Naghavi, Kato et al.: "MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein." in: Journal of biomedical science, Vol. 9, Issue 3, pp. 234-45, 2002 (PubMed).