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ATF4 antibody (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67)) (AA 170-198)

Details for Product anti-ATF4 Antibody No. ABIN950550, Supplier: Log in to see
Antigen
  • Atf-4
  • atf-4
  • ATF4
  • atf4
  • ATF4C
  • C/ATF
  • CREB-2
  • creb-2
  • CREB2
  • creb2
  • sb:eu681
  • TAXREB67
  • taxreb67
  • TXREB
  • txreb
  • wu:fb08f07
  • wu:fj01c08
  • wu:fk30b04
  • zgc:85951
  • zgc:171702
Epitope
AA 170-198, Middle Region
53
36
28
28
15
13
13
9
8
7
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
255
75
74
5
3
2
2
1
1
1
Host
Rabbit
211
47
15
Clonality
Polyclonal
Conjugate
This ATF4 antibody is un-conjugated
11
11
10
9
9
9
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
190
106
94
41
26
25
22
14
5
5
4
2
1
Supplier
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Immunogen KLH conjugated synthetic peptide between 170-198 amino acids from the Central region of human ATF4
Isotype Ig
Specificity This antibody reacts to ATF4.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography on Protein A
Alternative Name ATF4 (ATF4 Antibody Abstract)
Background This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.Synonyms: ATF-4, Activating transcription factor 4, CREB-2, CREB2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic AMP-responsive element-binding protein 2, DNA-binding protein TAXREB67, TXREB, cAMP-responsive element-binding protein 2
Gene ID 468
NCBI Accession NP_001666
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-ATF4 antibody (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67)) (AA 170-198) (ABIN950550) ATF4 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35µg/lan...
Background publications Dey, Baird, Zhou, Palam, Spandau, Wek: "Both transcriptional regulation and translational control of ATF4 are central to the integrated stress response." in: The Journal of biological chemistry, Vol. 285, Issue 43, pp. 33165-74, 2010 (PubMed).

Frank, Ge, Xie, Zhou, Tsai: "Control of activating transcription factor 4 (ATF4) persistence by multisite phosphorylation impacts cell cycle progression and neurogenesis." in: The Journal of biological chemistry, Vol. 285, Issue 43, pp. 33324-37, 2010 (PubMed).

Rzymski, Milani, Pike, Buffa, Mellor, Winchester, Pires, Hammond, Ragoussis, Harris: "Regulation of autophagy by ATF4 in response to severe hypoxia." in: Oncogene, Vol. 29, Issue 31, pp. 4424-35, 2010 (PubMed).

Liew, Bochenski, Kawamori, Hu, Leech, Wanic, Malecki, Warram, Qi, Krolewski, Kulkarni: "The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells." in: The Journal of clinical investigation, Vol. 120, Issue 8, pp. 2876-88, 2010 (PubMed).

Wek, Staschke: "How do tumours adapt to nutrient stress?" in: The EMBO journal, Vol. 29, Issue 12, pp. 1946-7, 2010 (PubMed).