EIF3K
Reactivity: Human
WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS, 0.09 % (W/V) sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
López-González, Schlüter, Aso, Garcia-Esparcia, Ansoleaga, LLorens, Carmona, Moreno, Fuso, Portero-Otin, Pamplona, Pujol, Ferrer: "Neuroinflammatory signals in Alzheimer disease and APP/PS1 transgenic mice: correlations with plaques, tangles, and oligomeric species." in: Journal of neuropathology and experimental neurology, Vol. 74, Issue 4, pp. 319-44, (2015) (PubMed).
Zhou, Sandercock, Fraser, Ridlova, Stephens, Schenauer, Yokoi-Fong, Barsky, Leary, Hershey, Doudna, Robinson: "Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18139-44, (2008) (PubMed).
Lin, Chen, Lin, Wang, Inoko, Inagaki, Wu, Chen: "eIF3k regulates apoptosis in epithelial cells by releasing caspase 3 from keratin-containing inclusions." in: Journal of cell science, Vol. 121, Issue Pt 14, pp. 2382-93, (2008) (PubMed).
Damoc, Fraser, Zhou, Videler, Mayeur, Hershey, Doudna, Robinson, Leary: "Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 7, pp. 1135-46, (2007) (PubMed).
Masutani, Sonenberg, Yokoyama, Imataka: "Reconstitution reveals the functional core of mammalian eIF3." in: The EMBO journal, Vol. 26, Issue 14, pp. 3373-83, (2007) (PubMed).
Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).
Schweizer, Bowden, Coulombe, Langbein, Lane, Magin, Maltais, Omary, Parry, Rogers, Wright: "New consensus nomenclature for mammalian keratins." in: The Journal of cell biology, Vol. 174, Issue 2, pp. 169-74, (2006) (PubMed).
Target
EIF3K
(Eukaryotic Translation Initiation Factor 3 Subunit K (EIF3K))
The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).Synonyms: Eukaryotic translation initiation factor 3 subunit 12, Eukaryotic translation initiation factor 3 subunit K, Muscle-specific gene M9 protein, PLAC-24, eIF-3 p25, eIF-3 p28