Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody (PE)

Details for Product No. ABIN953251
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms A830040C14Rik, AC-3, Apopain, CC3, CPP32, Caspase-3, Lice, Yama, mldy, Ly-6A.2, Ly-6A/E, Ly-6E.1, Sca-1, Sca1, TAP
Reactivity
Mouse (Murine)
(171), (24), (3)
Host
Rat
(148), (25), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
PE
(27), (21), (18), (14), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(149), (48), (45), (24), (10), (10), (6), (5), (4), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 0.3 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Clone CT-6A-6E
Isotype IgG2b
Specificity This monoclonal antibody recognizes Sca-1 (Ly-6A.2/6E.1), a cell surface antigen used in the identification of hematopoietic stem cells.
Purification Affinity chromatography on Protein G
Alternative Name Ly6A/E / SCA1 (Ly6a Antibody Abstract)
Background Ly6A/E is a member of the Ly-6 antigen family. The Thy-1lo, Lin- (lineage-negative, not expressing B220, Gr-1, Mac-1, CD4 or CD8), Sca-1+ population of bone marrow cells are highly purified, perhaps homogenous, pluripotent stem cells. This antigen is also present on various other tissues. Specific staining of the parenchymal cells can be demonstrated in thymus, spleen and kidney where as only vasculature reacts with anti-Sca-1 in brain, heart and liver (and possibly in lung). Also, Sca-1 is a T cell activation antigen, as surface expression of the antigen increases upon Con A activation of T lymphocytes. Sca-1 appears to have a molecular mass of 8 kDa under non-reducing conditions and 18 kDa under reducing conditions, indicating the presence of intra-chain disulfide bonds.Synonyms: Ly-6A.2/Ly-6E.1, Lymphocyte antigen 6A-2/6E-1, SCA-1, Stem cell antigen 1, T-cell-activating protein, TAP
Gene ID 110454
NCBI Accession NP_034868
UniProt P05533
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0,1 mg/mL
Buffer PBS containing 0.02 % sodium azide (NaN3) as preservative A highly purified grade of BSA has been added as a stablizing protein to bring the final protein concentration to 4-5 mg/mL after conjugation.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Aihara, Bühring, Aihara et al.: "An attempt to produce \pre-T\" cell hybridomas and to identify their antigens."" in: European journal of immunology, Vol. 16, Issue 11, pp. 1391-9, 1987 (PubMed).

Tomonari: "A rat antibody against a structure functionally related to the mouse T-cell receptor/T3 complex." in: Immunogenetics, Vol. 28, Issue 6, pp. 455-8, 1988 (PubMed).

Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, 1988 (PubMed).

van de Rijn, Heimfeld, Spangrude et al.: "Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 12, pp. 4634-8, 1989 (PubMed).

Codias, Cray, Baler et al.: "Expression of Ly-6A/E alloantigens in thymocyte and T-lymphocyte subsets: variability related to the Ly-6a and Ly-6b haplotypes." in: Immunogenetics, Vol. 29, Issue 2, pp. 98-107, 1989 (PubMed).

Validation Images
Catalog No. ABIN953251
1,666.50 $
Plus shipping costs $45.00
Quantity
Price
0.3 mg
1,666.50 $

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)
Did you look for something else?