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Mediator Complex Subunit 6 (MED6) (AA 134-164), (Middle Region) antibody

Details for Product No. ABIN953376, Supplier: Log in to see
Antigen
  • NY-REN-28
  • wu:fa14c11
  • zgc:86933
  • 1500012F11Rik
  • AU019660
  • AV213303
  • RNA polymerase transcriptional regulation mediator-related
  • mediator complex subunit 6
  • mediator of RNA polymerase II transcription, subunit 6 homolog (yeast)
  • RNA polymerase II transcriptional regulation mediator
  • AT3G21350
  • MED6
  • Med6
  • med6
Alternatives
anti-Human Mediator Complex Subunit 6 antibody for ELISA
Epitope
AA 134-164, Middle Region
15
10
10
9
9
8
8
7
5
2
2
2
1
1
Reactivity
Human
73
13
11
5
4
4
3
2
2
1
1
1
1
Host
Rabbit
60
14
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
70
37
3
2
2
1
1
1
Options
Supplier
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Immunogen KLH conjugated synthetic peptide between 134-164 amino acids from the Central region of human MED6
Isotype Ig
Specificity This antibody recognizes Human MED6 (Center).
Purification Protein A column, followed by peptide affinity purification
Alternative Name MED6 (MED6 Antibody Abstract)
Background Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.Synonyms: ARC33, Activator-recruited cofactor 33 kDa component, Mediator complex 6, Mediator of RNA polymerase II transcription subunit 6, Renal carcinoma antigen NY-REN-28
Molecular Weight 28425 Da
Gene ID 10001
NCBI Accession NP_005457
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Mediator Complex Subunit 6 (MED6) (AA 134-164), (Middle Region) antibody (ABIN953376) Western blot analysis of MED6 Antibody (Center) in WiDr cell line lysates (35ug/lane)...
Background publications Pavri, Lewis, Kim, Dilworth, Erdjument-Bromage, Tempst, de Murcia, Evans, Chambon, Reinberg: "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator." in: Molecular cell, Vol. 18, Issue 1, pp. 83-96, 2005 (PubMed).

Sato, Tomomori-Sato, Parmely, Florens, Zybailov, Swanson, Banks, Jin, Cai, Washburn, Conaway, Conaway: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).

Yang, DeBeaumont, Zhou, Näär: "The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 8, pp. 2339-44, 2004 (PubMed).

Tomomori-Sato, Sato, Parmely, Banks, Sorokina, Florens, Zybailov, Washburn, Brower, Conaway, Conaway: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).

Sato, Tomomori-Sato, Banks, Parmely, Sorokina, Brower, Conaway, Conaway: "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49671-4, 2003 (PubMed).