SETD2 antibody (Internal Region)

Catalog No. ABIN954755

Product Details anti-SETD2 Antibody

Target: SETD2 (SET Domain Containing 2 (SETD2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SETD2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-ERDPDKQTQN KE

Specificity: This antibody recognizes HYPB / SETD2 (internal region).

Cross-Reactivity (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_054878.3. Genename: SETD2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SETD2

Target: SETD2 (SET Domain Containing 2 (SETD2))

Alternative Name: SETD2 / HIF1 (SETD2 Products)

Synonyms: Kif9 antibody, HBP231 antibody, HIF-1 antibody, HIP-1 antibody, HYPB antibody, KMT3A antibody, SET2 antibody, p231HBP antibody, SET domain containing 2 antibody, Setd2 antibody, SETD2 antibody

Background: SET2 is a Histone methyltransferase that methylates 'Lys-36' of Histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. This protein probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. SET2 binds DNA at promoters, and may act as a transcription activator. SET2 binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. SET2 belongs to a class of huntingtin interacting proteins characterized by WW motifs.Synonyms: HIF-1, HIP-1, HIP1, HYPB, Histone-lysine N-methyltransferase SETD2, Huntingtin yeast partner B, Huntingtin-interacting protein 1, Huntingtin-interacting protein B, KIAA1732, KMT3A, Lysine N-methyltransferase 3A, SET domain-containing protein 2, SET2, p231HBP

Gene ID: 29072

NCBI Accession: NP_054878

Pathways: Tube Formation