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Details for Product No. ABIN955149

Ly-76 / TER-119 antibody (FITC)

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Antigen
Reactivity
»Alternatives Mouse (Murine)
Host
Clonality (Clone) Monoclonal ()
Conjugate
Application
»Alternatives Flow Cytometry (FACS)
Pubmed 8 references available
Catalog no. ABIN955149
Quantity 0.1 mg
Price
242.00 $   Plus shipping costs $45.00
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Immunogen Day 14 BALB/c fetal liver cells from Wistar Rat spleen.
Clone TER-119
Isotype IgG2b
Specificity Mouse Erythroid Cells (Ly-76). This anti-Mouse Erythroid cell (Ly-76) Monoclonal Antibody is selectively reactive with both fetal and adult erythroid cells. This monoclonal antibody (clone: TER119) is specific for cells at stages from early proerythroblast to mature erythrocytes. TER119 is reported to react with 20-25% of bone marrow cells and 2-3% of spleen cells but not with thymocytes or lymph node cells. In fetal haematopoietic tissues, 30-40% of day 10 yolk sac cells, 80-90% of day 14 fetal liver cells and 40-50% of newborn liver cells were reactive with AM31858FC. TER119+ cells in adult bone marrow expressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B cell (B220) markers. This Monoclonal Antibody immunoprecipitated protein bands with molecular weights of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane whereas only a 52 kDa band was detected by TER119 in Western Blot analysis. It has been determined that the TER119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself. Also the antigen is only expressed on normal erythroid cells but not on erythroleukaemia cells.
Purification Protein G Affinity Chromatography.
Background Alternate names: Lymphocyte antigen 76, TER119
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Ikuta, Kina, MacNeil et al.: "A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells." in: Cell, Vol. 62, Issue 5, pp. 863-74, 1990 (PubMed).

Randall, Weissman: "Characterization of a population of cells in the bone marrow that phenotypically mimics hematopoietic stem cells: resting stem cells or mystery population?" in: Stem cells (Dayton, Ohio), Vol. 16, Issue 1, pp. 38-48, 1998 (PubMed).

Suwabe, Takahashi, Nakano et al.: "GATA-1 regulates growth and differentiation of definitive erythroid lineage cells during in vitro ES cell differentiation." in: Blood, Vol. 92, Issue 11, pp. 4108-18, 1999 (PubMed).

Yanai, Matsui, Matsuda et al.: "A novel stromal cell-dependent hematopoietic cell line established from temperature-sensitive SV40 T-antigen transgenic mice." in: Experimental hematology, Vol. 27, Issue 6, pp. 1087-96, 1999 (PubMed).

Mukouyama, Chiba, Mucenski et al.: "Hematopoietic cells in cultures of the murine embryonic aorta-gonad-mesonephros region are induced by c-Myb." in: Current biology : CB, Vol. 9, Issue 15, pp. 833-6, 1999 (PubMed).

Kina, Ikuta, Takayama et al.: "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." in: British journal of haematology, Vol. 109, Issue 2, pp. 280-7, 2000 (PubMed).

Plant, Tobias: "Increased bone morphogenetic protein-6 expression in mouse long bones after estrogen administration." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 17, Issue 5, pp. 782-90, 2002 (PubMed).

Hartner, Schmittwolf, Kispert et al.: "Liver disintegration in the mouse embryo caused by deficiency in the RNA-editing enzyme ADAR1." in: The Journal of biological chemistry, Vol. 279, Issue 6, pp. 4894-902, 2004 (PubMed).

Alternatives for antigen "Ly-76 / TER-119", type "Antibodies"
Hosts (6)
Reactivities (6)
Applications (6), (3), (3), (3), (1)
Conjugates (2), (2), (1)
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