Western Blotting (WB), ELISA, Immunodiffusion (ID)
Specificity
Human Beta-2 Glycoprotein 1. In bidimensional immunoelectrophoresis a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Usage: This product is intended for use in precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell) to identify the presence of beta-2 glycoprotein 1 or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Sterile distilled water 1 ml
Concentration
Lot specific
Buffer
Lyophilized antiserum
Handling Advice
Avoid repeat freeze-thaw cycles.
Storage
4 °C,-20 °C
Storage Comment
Long term: -20°C Short term: +4°C. Avoid repeat freeze-thaw cycles.
Target
APOH
(Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))