MUC5B antibody (N-Term)

Catalog No. ABIN962141

Product Details anti-MUC5B Antibody

Target: MUC5B (Mucin 5 Subtype B (MUC5B))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC5B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Specific for the calculated ~590kD MUC5B protein. On Western Blots, recognizes a band significantly higher than 250kD. Reactivity has been confirmed with deglycosylated and secreted media from primary human tracheobranchial epithelial culture and a deglycosylated human saliva sample. Immunohistochemical assays were also performed using trachea samples from both normal and chronic obstructive pulmonary disease patients.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the N-terminal region of the human MUC5B protein.

Type of Immunogen: Synthetic peptide

Clone: 5B19-2E

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P, WB (5 - 10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Western Blot: 5-10 μg/mL. Immunohistochemistry (Formalin paraffin): 5-10 μg/mL. Note: Western Blot and Immunohistochemistry require deglycosylation. Refer to protocols.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for MUC5B

Target: MUC5B (Mucin 5 Subtype B (MUC5B))

Alternative Name: MUC5B (MUC5B Products)

Synonyms: MG1 antibody, MUC-5B antibody, MUC5 antibody, MUC9 antibody, RGD1561983 antibody, MUC5B antibody, 2300002I04Rik antibody, A130042M24 antibody, AV085033 antibody, mucin 5b antibody, mucin 5B, oligomeric mucus/gel-forming antibody, ovomucin, alpha subunit antibody, mucin 5AC, oligomeric mucus/gel-forming antibody, mucin-5B antibody, mucin 5, subtype B, tracheobronchial antibody, MUC5B antibody, Muc5b antibody, LOC395381 antibody, MUC5AC antibody, muc5b antibody, LOC100498539 antibody

Background: Name/Gene ID: MUC5B

Synonyms: MUC5B, MUC-5B, MUC9, Mucin-5B, Sublingual gland mucin, MG1, Cervical mucin, Cervical mucin MUC5B, MUC5

Gene ID: 727897

UniProt: Q9HC84