Connexin 31 antibody (N-Term)
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- Target See all Connexin 31 (GJB3) Antibodies
- Connexin 31 (GJB3) (Gap Junction Protein, beta 3, 31kDa (GJB3))
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Binding Specificity
- N-Term
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Connexin 31 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Specificity
- Recognizes mouse Connexin 31. Species sequence homology: shows 3 aa differences in rat Cx 31. The Cx31 peptide sequence does not significant homology with other known Connexins.
- Purification
- Immunoaffinity purified
- Immunogen
- A 17 amino acid peptide sequence within the 1st cytoplasmic domain of the rat Cx31 (2) was used to generate anti-Mouse Cx31. Amino terminal Cysteine was added for conjugation to KLH (carrier protein).
- Isotype
- IgG
- Top Product
- Discover our top product GJB3 Primary Antibody
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- Application Notes
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Approved: ELISA (1:100), IHC (2 - 20 μg/mL), WB (1 - 10 μg/mL)
Usage: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Western Blot: 1-10 μg/mL using ECL. An antibody raised against a GST-fusion has been shown to recognize ~31kD band in skin. Immunohistochemistry: 2-20 μg/mL in formaldehyde fixed tissues. ELISA: 1:100K using 50-100 ng CX31 control peptide/well. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in 100 μL of Sterile PBS.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, 0.05 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- Connexin 31 (GJB3) (Gap Junction Protein, beta 3, 31kDa (GJB3))
- Alternative Name
- GJB3 / CX31 / Connexin 31 (GJB3 Products)
- Synonyms
- CX31 antibody, DFNA2 antibody, DFNA2B antibody, EKV antibody, Cnx31 antibody, Cx31 antibody, D4Wsu144e antibody, Gjb-3 antibody, ekv antibody, cx31 antibody, dfna2 antibody, gjb3b antibody, dfna2b antibody, connexin-31 antibody, gap junction protein beta 3 antibody, gap junction protein, beta 3 antibody, gap junction protein beta 3 L homeolog antibody, GJB3 antibody, Gjb3 antibody, gjb3.L antibody, gjb3 antibody
- Background
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Name/Gene ID: GJB3
Subfamily: Connexin
Family: Ion Channel
Synonyms: GJB3, Connexin 31, Connexin-31, CX31, DFNA2, Erythrokeratodermia variabilis, Gap junction beta-3 protein, DFNA2B, EKV, Gap junction protein beta 3 - Gene ID
- 2707
- UniProt
- O75712
- Pathways
- Sensory Perception of Sound
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