V5 Epitope Tag antibody

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Antigen
Reactivity
Tag
404
3
3
3
3
Host
Mouse
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135
37
9
5
4
3
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
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27
19
11
10
10
9
9
9
8
8
8
8
8
8
7
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
342
265
122
91
71
64
53
35
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Options
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Brand THE™
Immunogen V5 tag peptide conjugated - KLH
Sequence GKPIPNPLLG LDST
Clone 4C12E11
Isotype IgG2a
Specificity THETM V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
Characteristics THETM V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.
Purification Protein A affinity column
Alternative Name V5 Tag
Target Type Tag
Background The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
Research Area Tags/Labels
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/mL
Western blot: 0.2-1 µg/mL
Immunoprecipitation: 1-5 µg/mL
FACS: 1-3 µg for 1 x 10^6 cells
ICC/IF: 1-3 µg/mL
Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Product cited in: Liew, Assmann, Templin, Raum, Lipson, Rajan, Qiang, Hu, Kawamori, Lindberg, Philipson, Sonenberg, Goldfine, Stoffers, Mirmira, Urano, Kulkarni: "Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic ? cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 22, pp. E2319-28, 2014 (PubMed).

Wang, Li, Felsovalyi, Chen, Cardozo, Krogsgaard: "Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers." in: ACS chemical biology, Vol. 9, Issue 9, pp. 2165-72, 2014 (PubMed).

Kim, Li, Ahn, Song, Zhang: "Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity." in: Scientific reports, Vol. 4, pp. 7330, 2014 (PubMed).

Van Gorp, Van Breedam, Van Doorsselaere, Delputte, Nauwynck: "Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus." in: Journal of virology, Vol. 84, Issue 6, pp. 3101-5, 2010 (PubMed).