Goat anti-Rat IgM (Heavy & Light Chain) Antibody (PE) - Preadsorbed
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- Target See all IgM products
- IgM
- Fragment
- F(ab')2 fragment
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- PE
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Application
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgM, and Rat Serum.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Rat IgM whole molecule
- Isotype
- IgG
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- Application Notes
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Application Note: F(ab')2 Anti-Rat IgM Antibody Phycoerythrin conjugation is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Flow Cytometry Dilution: 1:100 - 1:250
IF Microscopy Dilution: 1:100 - 1:250
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 0.5 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing. - Storage
- RT,4 °C
- Storage Comment
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
- Expiry Date
- 12 months
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- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
- Background
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Synonyms: Goat F(ab')2 Anti-Rat IgM (mu chain) Phycoerythrin Conjugated Antibody, Goat Fab2 Anti-Rat IgM mu chain Antibody PE Conjugation
Background: F(ab')2 Anti-Rat IgM Antibody generated in goat detects reactivity to Rat IgM. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. This F(ab')2 Anti-Rat IgM Antibody is conjugated to Phycoerythrin.
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