CD43B antibody
Reactivity: Human
IHC (fp), IHC (fro), FACS, IF
Host: Mouse
Monoclonal
B310 (BRA-7G)
unconjugated
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- Target
- CD43B
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
- Specificity
- This antibody reacts with a 95-115 kD glycoprotein on T-cells and thymocytes and a 115-135 kD molecule on neutrophils and platelets.
- Purification
- Purified.
- Immunogen
- Immature pluripotential human leukemia cell line K562.
- Clone
- B310 (BRA-7G)
- Isotype
- IgM
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- Application Notes
-
Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Westernblotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-20 ug/mL in IHC. - Comment
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Recommended Positive Control: Tonsil and Lymph Node
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.4 mg/ml.
- Buffer
- 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
-
- Target
- CD43B
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