CDK9 antibody

Catalog No. ABIN965846

There are 2+ publications for this product available. The Mouse Monoclonal anti-CDK9 antibody is suitable to detect CDK9 in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for CDK9 antibody (ABIN965846)

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 1B5D10

Product Details anti-CDK9 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CDK9 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CDK9 antibody (ABIN965846)

Montanuy, Torremocha, Hernández-Munain, Suñé: "Promoter influences transcription elongation: TATA-box element mediates the assembly of processive transcription complexes responsive to cyclin-dependent kinase 9." in: The Journal of biological chemistry, Vol. 283, Issue 12, pp. 7368-78, (2008) (PubMed).

Sabò, Lusic, Cereseto, Giacca: "Acetylation of conserved lysines in the catalytic core of cyclin-dependent kinase 9 inhibits kinase activity and regulates transcription." in: Molecular and cellular biology, Vol. 28, Issue 7, pp. 2201-12, (2008) (PubMed).

Target Details for CDK9

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Alternative Name: CDK9

Background: The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Tissue specificity: Ubiquitous.
Synonyms: TAK, C-2k, CTK1, CDC2L4, PITALRE

Molecular Weight: 43kDa

Gene ID: 1025

Pathways: Cell Division Cycle