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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Checkpoint Kinase 1 (CHEK1) antibody
|Synonyms||CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1|
Alternatives Western Blotting (WB), ELISA
|2 references available|
|Price||330.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 5 to 7 Business Days|
|Description||Active recombinant full length human CHK1 containing an Nterminal GST tag. This protein has an apparent molecular weight of ~82kDa. CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia.|
|Specificity||Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15.|
Western Blot: Dilution 1: 2,00- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
|Purification||Antibodies are purified by protein G affinity chromatography.|
|Storage||Store at -20° C.|
|Research Area||Kinases/Phosphatases, Cell Cycle|
|Restrictions||For Research Use only|
OConnell, Raleigh, Verkade et al.: "Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation." in: The EMBO journal, Vol. 16, Issue 3, pp. 545-54, 1997 (PubMed).
Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).