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Checkpoint Kinase 1 (CHEK1) antibody

Antigen

Checkpoint Kinase 1 (CHEK1)
Synonyms CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1
Clonality Monoclonal
Host
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), ELISA
2 references available
Catalog no. ABIN965882
Quantity 0.1mg
Price 330.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name CHK1
Isotype IgG1
Description Active recombinant full length human CHK1 containing an Nterminal GST tag. This protein has an apparent molecular weight of ~82kDa. CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia.
Specificity Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15.

Application Details
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Application Notes Western Blot: Dilution 1: 2,00- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Purification Antibodies are purified by protein G affinity chromatography.
Storage Store at -20° C.
Research Area Kinases/Phosphatases, Cell Cycle
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product OConnell, Raleigh, Verkade et al.: "Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation." in: The EMBO journal, Vol. 16, Issue 3, pp. 545-54, 1997 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Checkpoint Kinase 1 (CHEK1)", type "Antibodies"
Hosts Rabbit (226), Mouse (20), Goat (6), Sheep (4)
Reactivities Human (243), Rat (Rattus) (131), Mouse (Murine) (124), Horse (Equine) (36), Cow (Bovine) (22), Chicken (21), Dog (Canine) (20), Pig (Porcine) (19), Monkey (4), Xenopus laevis (2), Yeast (2), Zebrafish (2), Cat (Feline) (1), Rabbit (1), Yeast (Saccharomyces cerevisiae) (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (149), ELISA (92), Immunofluorescence (IF) (91), Immunohistochemistry (IHC) (46), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (44), Immunoprecipitation (IP) (18), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (15), Immunocytochemistry (ICC) (10), Immunoelectron Microscopy (IEM) (5), Dot Blot (DB) (2), Dot Blot (Dot) (2), Flow Cytometry (FACS) (2)
Conjugates Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), Biotin (5), Cy3 (5), Cy5 (5), Cy5.5 (5), Cy7 (5), FITC (5), Gold (5), HRP (5), PE (5), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
Epitopes pSer317 (37), pSer280 (34), pSer345 (30), Ser296 (18), Internal Region (7), C-Term (6), pSer296 (6), Ser280 (4), AA 312-327 (3), N-Term (3), pSer296,Internal Region (3), Center (2), AA 1-474 (1), AA 250-300 (1), AA 259-311 (1), Internal Region,AA 251-279 (1), Middle Region (1), pSer286 (1), pSer301 (1)