Checkpoint Kinase 1 (CHEK1) antibody

Details for Product No. ABIN965882
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Antigen
Synonyms An08g10320, AO090003000441, CHK1, chk1, C85740, Chk1, rad27, id:ibd2720, zgc:56093, CHEK1
Reactivity
Human
(395), (133), (125), (36), (26), (26), (14), (4), (3), (2), (2), (2), (1)
Host
(348), (39), (12), (7)
Clonality
Monoclonal
Conjugate
Un-conjugated
(9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4)
Application
Western Blotting (WB), ELISA
(310), (157), (99), (58), (50), (50), (26), (21), (11), (4), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN965882
343.75 $
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Isotype IgG1
Specificity Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15.
Purification Antibodies are purified by protein G affinity chromatography.
Alternative Name CHK1
Background Active recombinant full length human CHK1 containing an Nterminal GST tag. This protein has an apparent molecular weight of ~82kDa. CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia.
Research Area Kinases/Phosphatases, Cell Cycle
Application Notes Western Blot: Dilution 1: 2,00- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Storage -20 °C
Product cited in: OConnell, Raleigh, Verkade et al.: "Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation." in: The EMBO journal, Vol. 16, Issue 3, pp. 545-54, 1997 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

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