CHEK2 antibody (AA 481-531)

Catalog No. ABIN965887

This Mouse Monoclonal antibody specifically detects CHEK2 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for CHEK2 antibody (AA 481-531) (ABIN965887)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 1C12B8

Product Details anti-CHEK2 Antibody

Binding Specificity: AA 481-531

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CHEK2 antibody (ABIN965887)

Nat, Nilbratt, Narkilahti, Winblad, Hovatta, Nordberg: "Neurogenic neuroepithelial and radial glial cells generated from six human embryonic stem cell lines in serum-free suspension and adherent cultures." in: Glia, Vol. 55, Issue 4, pp. 385-99, (2007) (PubMed).

Antoni, Sodha, Collins, Garrett: "CHK2 kinase: cancer susceptibility and cancer therapy - two sides of the same coin?" in: Nature reviews. Cancer, Vol. 7, Issue 12, pp. 925-36, (2007) (PubMed).

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: CHK2

Background: CHK2: CHK2 checkpoint homolog (S. pombe). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with LiFraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Synonyms: CDS1, LFS2, CHEK2

Molecular Weight: 61kDa

Gene ID: 11200

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle