Cytokeratin 8 (52.2 kDa) antibody

Antigen: Cytokeratin 8 (52.2 kDa)

Clonality: Monoclonal (B391)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN965966

Additional Information

Immunogen: Cytoskeleton preparation from HeLa Cells

Isotype: IgG1

Clone: B391

Description: This antibody reacts with Cytokeratin 8 (52 and 45 kD) human polypeptide. This cytokeratin coexists With Cytokeratin 18 and is found in all non-squamous epithelium but not in squamous epithelium. It Reacts with the adenocarcinomas of ovary, gastrointestinal tract, prostate, and thyroid.

Comments:

Cellular Localization: Cytoplasm.
Recommended Positive Control: Colon, Lung, or Breast Carcinomas

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen): 1:50 -1:100.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with AutoProbe III. The optimal conditions should be determined by the individual laboratory. The Auto/Zyme predigestion or high temperature antigenic unmasking technique is recommended with this antibody when using paraffinembedded tissue sections.

Concentration: 35 mg/ml

Purification: Concentrated.

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: 0.05% Sodium Azide

Storage: Store at 2-8° C for short term, freeze under -20°C for long term storage.

Restrictions: For Research Use only

Publications

Product: Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).