Chemokine (C-C Motif) Ligand 2 (CCL2) (N-Term,AA 24-36) antibody

Antigen: Chemokine (C-C Motif) Ligand 2 (CCL2)

Synonyms: HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, JE, Scya2, Sigje, AI323594, CCL2, DKFZp469C111

Binding Site: N-Term,AA 24-36

Clonality: Polyclonal

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN966546

Additional Information

Alternative name: MCP-1

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human MCP-1 (24-36 aa), different from the mouse sequence by two amino acids.

Reconstitution: 0.2ml of distilled water will yield a concentration of 500µg/ml.

Description: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3 Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Application Details

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative: 0.05mg Thiomersal, 0.05mg NaN3.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month.

Research Area: Chemokines

Restrictions: For Research Use only

Publications

Product: Furutani, Nomura, Notake et al.: "Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF)." in: Biochemical and biophysical research communications, Vol. 159, Issue 1, pp. 249-55, 1989 (PubMed).

Gu, Okada, Clinton et al.: "Absence of monocyte chemoattractant protein-1 reduces atherosclerosis in low density lipoprotein receptor-deficient mice." in: Molecular cell, Vol. 2, Issue 2, pp. 275-81, 1998 (PubMed).

Gosling, Slaymaker, Gu et al.: "MCP-1 deficiency reduces susceptibility to atherosclerosis in mice that overexpress human apolipoprotein B." in: The Journal of clinical investigation, Vol. 103, Issue 6, pp. 773-8, 1999 (PubMed).