MEK1 antibody

Catalog No. ABIN966563

There are 4+ publications for this product available. The Rabbit Polyclonal anti-MEK1 antibody is suitable to detect MEK1 in samples from Human, Mouse and Rat. It has been validated for IHC.

Quick Overview for MEK1 antibody (ABIN966563)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-MEK1 Antibody

Specificity: MEK1 (Ab-221) antibody detects endogenous levels of total MEK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human MEK1 around the phosphorylation site of serine 221 (A-N-SP-F-V).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-MEK1 antibody (ABIN966563)

Zebisch, Staber, Delavar, Bodner, Hiden, Fischereder, Janakiraman, Linkesch, Auner, Emberger, Windpassinger, Schimek, Hoefler, Troppmair, Sill: "Two transforming C-RAF germ-line mutations identified in patients with therapy-related acute myeloid leukemia." in: Cancer research, Vol. 66, Issue 7, pp. 3401-8, (2006) (PubMed).

Luciano, Hsu, Channavajhala, Lin, Cuozzo: "Phosphorylation of threonine 290 in the activation loop of Tpl2/Cot is necessary but not sufficient for kinase activity." in: The Journal of biological chemistry, Vol. 279, Issue 50, pp. 52117-23, (2004) (PubMed).

Gopalbhai, Jansen, Beauregard, Whiteway, Dumas, Wu, Meloche: "Negative regulation of MAPKK by phosphorylation of a conserved serine residue equivalent to Ser212 of MEK1." in: The Journal of biological chemistry, Vol. 278, Issue 10, pp. 8118-25, (2003) (PubMed).

Wang, Jin, Wong, Feng, Wong, Tsao: "MAD2-induced sensitization to vincristine is associated with mitotic arrest and Raf/Bcl-2 phosphorylation in nasopharyngeal carcinoma cells." in: Oncogene, Vol. 22, Issue 1, pp. 109-16, (2003) (PubMed).

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MEK1

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling