MEK1 antibody

Catalog No. ABIN966564

Cited in 4+ publications. The Rabbit Polyclonal anti-MEK1 antibody (ABIN966564) specifically detects MEK1 in IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MEK1 antibody (ABIN966564)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-MEK1 Antibody

Specificity: MEK1 (Ab-291) antibody detects endogenous levels of total MEK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human MEK1 around the phosphorylation site of threonine 291 (P-R-TP-P-G).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-MEK1 antibody (ABIN966564)

Burroughs, Oh, Barrett, DiAugustine et al.: "Phosphatidylinositol 3-kinase and mek1/2 are necessary for insulin-like growth factor-I-induced vascular endothelial growth factor synthesis in prostate epithelial cells: a role for hypoxia-inducible ..." in: Molecular cancer research : MCR, Vol. 1, Issue 4, pp. 312-22, (2003) (PubMed).

Schramek, Feifel, Marschitz, Golochtchapova, Gstraunthaler, Montesano: "Loss of active MEK1-ERK1/2 restores epithelial phenotype and morphogenesis in transdifferentiated MDCK cells." in: American journal of physiology. Cell physiology, Vol. 285, Issue 3, pp. C652-61, (2003) (PubMed).

Piatelli, Doughty, Chiles: "Requirement for a hsp90 chaperone-dependent MEK1/2-ERK pathway for B cell antigen receptor-induced cyclin D2 expression in mature B lymphocytes." in: The Journal of biological chemistry, Vol. 277, Issue 14, pp. 12144-50, (2002) (PubMed).

Morgan, Labno, Van Seventer, Denny, Straus, Burkhardt: "Superantigen-induced T cell:B cell conjugation is mediated by LFA-1 and requires signaling through Lck, but not ZAP-70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5708-18, (2001) (PubMed).

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MEK1

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling