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Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody

Antigen

Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)
Synonyms MEK1, MKK1, MAPKK1, PRKMK1, Mek1, MEKK1, Prkmk1
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN966565
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name MEK1
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T).
Specificity MEK2 (Ab-394) antibody detects endogenous levels of total MEK2 protein

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Cowley, Paterson, Kemp et al.: "Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells." in: Cell, Vol. 77, Issue 6, pp. 841-52, 1994 (PubMed).

Rosen, Ginty, Weber et al.: "Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras." in: Neuron, Vol. 12, Issue 6, pp. 1207-21, 1994 (PubMed).

Alessi, Saito, Campbell et al.: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)", type "Antibodies"
Hosts Rabbit (232), Mouse (12), Chicken (1)
Reactivities Human (234), Mouse (Murine) (130), Rat (Rattus) (126), Pig (Porcine) (25), Dog (Canine) (16), Horse (Equine) (15), Cow (Bovine) (14), Chicken (12), Xenopus laevis (5), Monkey (2)
Applications Western Blotting (WB) (166), ELISA (86), Immunohistochemistry (IHC) (61), Immunofluorescence (IF) (51), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (42), Immunoprecipitation (IP) (12), Immunocytochemistry (ICC) (8), Dot Blot (Dot) (7), Flow Cytometry (FACS) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (DB) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), PE,Cy5.5 (6), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pThr291 (10), pThr292 (10), pThr386 (10), N-Term (9), pSer217 (9), pSer221 (8), Internal Region (7), pSer298 (7), pThr286 (6), pThr386,C-Term (6), pSer385 (5), C-Term (4), pSer218, pSer222 (4), pSer218 (3), pSer222 (3), pSer298,Internal Region (3), Ser217 (2), Ser218 (2), Ser221 (2), Ser297 (2), Thr291 (2), Thr385 (2), pSer297 (2), pThr385 (2), AA 1-12 (1), C-Term,AA 353-367 (1), Center (1), Ser218,Ser222 (1), Thr285 (1), Thr386 (1), pSer217, pSer221 (1), pSer408 (1), pSer551 (1)