Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody

Details for Product No. ABIN966565
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Antigen
Synonyms
Mek1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEKK1, Prkmk1, mek-2, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, ATMEK1, F20B18.180, F20B18_180, MAP kinase/ ERK kinase 1, MITOGEN ACTIVATED PROTEIN  ... show more
Mek1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEKK1, Prkmk1, mek-2, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, ATMEK1, F20B18.180, F20B18_180, MAP kinase/ ERK kinase 1, MITOGEN ACTIVATED PROTEIN KINASE KINASE, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1, NMAPKK, MEK show less
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(407), (221), (211), (72), (46), (40), (33), (8), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(377), (41), (2), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (1), (1)
Application
Immunohistochemistry (IHC)
(329), (123), (103), (92), (60), (50), (21), (20), (14), (3), (2), (1)
Pubmed 4 references available
Quantity 100 µg
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Catalog No. ABIN966565
385.00 $
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Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T).
Specificity MEK2 (Ab-394) antibody detects endogenous levels of total MEK2 protein
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name MEK1
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Cowley, Paterson, Kemp et al.: "Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells." in: Cell, Vol. 77, Issue 6, pp. 841-52, 1994 (PubMed).

Rosen, Ginty, Weber et al.: "Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras." in: Neuron, Vol. 12, Issue 6, pp. 1207-21, 1994 (PubMed).

Alessi, Saito, Campbell et al.: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994 (PubMed).

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