Metadherin (MTDH) antibody

Details for Product No. ABIN966573, Supplier: Log in to see
Antigen
  • MGC75963
  • 2610103J23Rik
  • 3D3
  • 3D3/Lyric
  • AEG-1
  • AV353288
  • D8Bwg1112e
  • Lyric
  • AEG1
  • LYRIC
  • LYRIC/3D3
  • metadherin
  • mtdh
  • MTDH
  • Mtdh
Alternatives
anti-Human Metadherin antibody for ELISA
Reactivity
Human
98
33
29
3
1
1
1
1
1
Host
Mouse
55
42
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
85
44
34
33
26
13
11
6
5
2
1
1
1
Options
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Immunogen Purified recombinant fragment of human Metadherin expressed in E. Coli.
Clone 2F11C3
Isotype IgG1
Purification Antibodies are purified by protein G affinity chromatography.
Alternative Name Metadherin (MTDH Antibody Abstract)
Background Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain, blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
Synonyms: 3D3, AEG1, LYRIC, MTDH
Molecular Weight 64kDa
Gene ID 92140
Pathways Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization
Application Notes Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Hu, Chong, Yang, Wei, Blanco, Li, Reiss, Au, Haffty, Kang: "MTDH activation by 8q22 genomic gain promotes chemoresistance and metastasis of poor-prognosis breast cancer." in: Cancer cell, Vol. 15, Issue 1, pp. 9-20, 2008 (PubMed).

Méndez, Audicana, Cancer, Isern, Llaneza, Moreno, Navarro, Tarancón, Valero, Ribas, Jofre, Lucena: "Assessment of drinking water quality using indicator bacteria and bacteriophages." in: Journal of water and health, Vol. 2, Issue 3, pp. 201-14, 2004 (PubMed).

Britt, Yang, Yang, Flanagan, Callanan, Lim, Lin, Hixson: "Identification of a novel protein, LYRIC, localized to tight junctions of polarized epithelial cells." in: Experimental cell research, Vol. 300, Issue 1, pp. 134-48, 2004 (PubMed).

Brown, Ruoslahti: "Metadherin, a cell surface protein in breast tumors that mediates lung metastasis." in: Cancer cell, Vol. 5, Issue 4, pp. 365-74, 2004 (PubMed).