Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) antibody
| Antigen |
|
| Synonyms |
MEK3, MKK3, MAPKK3, PRKMK3, Prkmk3, mMKK3b, AW212142 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
Human (147), Rat (Rattus) (80), Mouse (Murine) (69), Dog (Canine) (17), Cow (Bovine) (16), Chicken (15), Horse (Equine) (13), Pig (Porcine) (13), Zebrafish (2), Cat (Feline) (1), Xenopus laevis (1)
|
|
Conjugate
|
Biotin (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy7 (3), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2)
|
|
Application
|
Western Blotting (WB) (105), Immunofluorescence (IF) (76), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (38), Immunohistochemistry (IHC) (26), Immunoprecipitation (IP) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunocytochemistry (ICC) (6), Immunoelectron Microscopy (IEM) (3), Dot Blot (Dot) (2), Flow Cytometry (FACS) (1)
|
| Catalog no. |
ABIN966579 |
| Quantity |
100ug (100ug/100ul.) (Variants) |
| Price |
385.00 $ Plus shipping costs $45.00
|
|
Bulk discount
|
| Shipping to |
|
| Availability |
Will be delivered in 5 to 7 Business Days |
|
Alternative name
|
MKK3
|
|
Immunogen
|
The antiserum was produced against synthesized non-phosphopeptide derived from human MKK3 around the phosphorylation site of serine189 (V-D-SP-V-A).
|
|
Specificity
|
MKK3 (Ab-189) antibody detects endogenous levels of total MKK3 protein.
|
|
Application Notes
|
WB: 1:500~1:1000
IHC: 1:50~1:100.
|
|
Concentration
|
100ug/100ul.
|
|
Purification
|
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|
|
Buffer
|
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
|
|
Preservative
|
0.02% Sodium Azide
|
|
Storage
|
Store at -20°C/1 year.
|
|
Restrictions
|
For Research Use only
|
|
Product
|
Raingeaud, Whitmarsh, Barrett et al.: "MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway." in: Molecular and cellular biology, Vol. 16, Issue 3, pp. 1247-55, 1996 (PubMed).
Wang, Chen, Liao et al.: "Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3389-403, 2002 (PubMed).
|
Alternatives for antigen "Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3)", type "Antibodies"
|
Hosts
|
Rabbit (120), Mouse (30)
|
|
Reactivities
|
Human (147), Rat (Rattus) (80), Mouse (Murine) (69), Dog (Canine) (17), Cow (Bovine) (16), Chicken (15), Horse (Equine) (13), Pig (Porcine) (13), Zebrafish (2), Cat (Feline) (1), Xenopus laevis (1)
|
|
Applications
|
Western Blotting (WB) (105), Immunofluorescence (IF) (76), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (38), Immunohistochemistry (IHC) (26), Immunoprecipitation (IP) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunocytochemistry (ICC) (6), Immunoelectron Microscopy (IEM) (3), Dot Blot (Dot) (2), Flow Cytometry (FACS) (1)
|
|
Conjugates
|
Biotin (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy7 (3), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2)
|
|
Epitopes
|
pSer189 (29), C-Term (19), pThr222 (19), Ser189 (6), N-Term (5), C-Term,AA 320-334 (3), pSer189,Internal Region (3), Internal Region (2), C-Term,AA 20-334 (1), Center (1), Internal Region,AA 131-160 (1), Ser222 (1)
|
Alternatives 
