Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody

Antigen

3-phosphoinositide Dependent Protein Kinase-1 (PDPK1)
Synonyms PDK1, PRO0461, MGC20087, MGC35290, Pdk1, PDPK1, pdpk1, MGC82080, B29, IGB, Igb, Igbeta, Ig-beta, MGC108607, CD79B, LOC100304995
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
5 references available
Catalog no. ABIN966816
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 5 to 7 Business Days

Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name PDK1 (Ab-241)
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241(A-N-SP-F-V).
Specificity PDK1 (Ab-241) antibody detects endogenous levels of total PDK1 protein.

Application Details
Publications Alternatives Variants back to top

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Chen, Nystrom, Dong et al.: "Insulin stimulates increased catalytic activity of phosphoinositide-dependent kinase-1 by a phosphorylation-dependent mechanism." in: Biochemistry, Vol. 40, Issue 39, pp. 11851-9, 2001 (PubMed).

Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, 2002 (PubMed).

Lim, Kikani, Wick et al.: "Nuclear translocation of 3'-phosphoinositide-dependent protein kinase 1 (PDK-1): a potential regulatory mechanism for PDK-1 function." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 24, pp. 14006-11, 2003 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Scheid, Parsons, Woodgett: "Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation." in: Molecular and cellular biology, Vol. 25, Issue 6, pp. 2347-63, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "3-phosphoinositide Dependent Protein Kinase-1 (PDPK1)", type "Antibodies"
Hosts Rabbit (89), Mouse (2), Goat (1)
Reactivities Human (89), Mouse (Murine) (31), Rat (Rattus) (27), Chicken (20), Cow (Bovine) (20), Dog (Canine) (20), Pig (Porcine) (19), Cat (Feline) (2), Rabbit (1), Zebrafish (1)
Applications ELISA (59), Western Blotting (WB) (57), Immunofluorescence (IF) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunohistochemistry (IHC) (11), Immunoprecipitation (IP) (6), Dot Blot (Dot) (5), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (5), pSer241 (5), AA 301-312 (2), Internal Region (2), N-Term (2), Ser241 (2), Ser393 (2), Ser396 (2), Ser410 (2), Tyr9 (2), pSer393 (2), pSer396 (2), pSer410 (2), pTyr9 (2), AA 29-436 (1), N-Term,His67 (1), N-Term,Pro71 (1), pSer, pThr (1)