CD72 B and C Alloantigens antibody

Details for Product No. ABIN967281
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Antigen
Reactivity
Mouse (Murine)
Host
Rat
Clonality (Clone)
Monoclonal ()
Application
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
Pubmed 8 references available
Catalog no. ABIN967281
Quantity 0.1 mg
Price
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Immunogen BALB/c spleen cells
Clone JY
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Background The JY/93 antibody reacts with Lyb-2.2 and Lyb-2.3 (CD72 b and c alloantigens, respectively). CD72 is a 45-kDa type-II membrane protein containing a C-type lectin-like domain, an ITIM and ITIM-like sequence in the cytoplasmic tail. Recently, CD72 has been shown to negatively regulate B-cell receptor signaling. Analysis of CD72-deficient mice supports these results and shows that CD72 is involved in B-cell development. CD72-stimulated B cells show a transient association of CD19 with CD72, as well as an increase in Tyr-phosphorylation of CD19. CD72 is reported to be a ligand for CD5,5 although this is controversial. The CD72 alloantigens Lyb-2.1 (originally identified as Ly-m19.2), Lyb-2.2 (originally identified as Ly-32.2), Lyb-2.3, and Lyb-2.4 are encoded by the Cd72[a], Cd72[b], Cd72[c] , and Cd72[d] alleles, respectively. Lyb-2.2 (CD72b) is expressed on B lymphocytes, a subset of peripheral T cells, activated thymocytes, and activated T lymphocytes in A, BALB/c, CBA/H, CBA/N, C3H/He, C57BL, NZB, NZW, PL, and 129 strains. Moreover, expression of CD72b on T cells is dominant in F1 hybrid mice, and its level of expression increases upon activation. Lyb-2.3 (CD72c) is expressed on B cells of AKR, CE, SJL, and other mouse strains. Although staining of a small percentage of splenic T cells has been detected in CD72c mice, northern blot analysis has shown no expression of CD72c in T lymphocytes of these mice. JY/93 mAb does not stain cells from CD72a mice (e.g., CBA/J, DBA/2, SWR), and it does not stain resting thymocytes, splenic CD11b-positive cells, or NK cells. Five serological specificities of CD72 alloantigens have been described and the nomenclature CD72.1, CD72.2, CD72.3, CD72.4, and CD72.5 proposed.
Synonyms: Lyb-2.2 and Lyb-2.3
Application Notes Mouse Fc Block™ should not be used when staining with JY/93 mAb. This antyibody has been tested by Western Blot during development on 2PK-3 cell lysate and it recognizes a 45kDa band. However, it is not tested each lot by Western Blot.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Product cited in: Luo, Van de Velde, von Hoegen et al.: "Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 6, pp. 1630-4, 1992 (PubMed).

Robinson, Ying, Miceli et al.: "Extensive polymorphism in the extracellular domain of the mouse B cell differentiation antigen Lyb-2/CD72." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 880-6, 1992 (PubMed).

Robinson, Landolfi, Schäfer et al.: "Biochemical identity of the mouse Ly-19.2 and Ly-32.2 alloantigens with the B cell differentiation antigen Lyb-2/CD72." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 9, pp. 4764-72, 1993 (PubMed).

Leong, Marley, Loh et al.: "The induction of immune responses to murine malignant mesothelioma by IL-2 gene transfer." in: Immunology and cell biology, Vol. 75, Issue 4, pp. 356-9, 1997 (PubMed).

Bikah, Lynd, Aruffo et al.: "A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5." in: International immunology, Vol. 10, Issue 8, pp. 1185-96, 1998 (PubMed).

Venkataraman, Lu, Buhl et al.: "CD72-mediated B cell activation involves recruitment of CD19 and activation of phosphatidylinositol 3-kinase." in: European journal of immunology, Vol. 28, Issue 10, pp. 3003-16, 1998 (PubMed).

Pan, Baumgarth, Parnes: "CD72-deficient mice reveal nonredundant roles of CD72 in B cell development and activation." in: Immunity, Vol. 11, Issue 4, pp. 495-506, 1999 (PubMed).

Adachi, Wakabayashi, Nakayama et al.: "CD72 negatively regulates signaling through the antigen receptor of B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 3, pp. 1223-9, 2000 (PubMed).

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