Interferon Regulatory Factor 3 (IRF3) (AA 56-427) antibody

Details for Product No. ABIN967285, Supplier: Log in to see
Antigen
  • irf3
  • IRF3
  • C920001K05Rik
  • IRF-3
  • interferon regulatory factor 3
  • interferon regulatory factor 7
  • irf3
  • IRF3
  • irf-3
  • Irf3
  • IRF7
Alternatives
anti-Human Interferon Regulatory Factor 3 antibody for Biochemical Assay
Epitope
AA 56-427
24
23
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15
15
13
13
12
9
5
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2
1
1
1
1
1
1
1
1
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1
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1
Reactivity
Human
274
102
58
6
6
6
3
2
2
1
1
1
1
Host
Mouse
212
86
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
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4
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2
2
2
2
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2
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
247
107
65
64
41
41
37
27
27
14
10
8
4
3
3
1
1
1
Options
Supplier
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Immunogen Human IRF-3 recombinant fusion protein
Clone SL-12-1
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name IRF-3 (IRF3 Antibody Abstract)
Background Viral infection in mammals can lead to the induction of multiple pathways as part of the host defense mechanism. One of the major pathways activated is the JAK-STAT pathway by various interferons (IFNalpha and IFNbeta). These IFNs exert their influence via transcriptional activation of specific target genes involved in antiviral defense, for example the chemokine ISG15 gene or the major histocompatibility complex class I and II molecules. These genes in turn are regulated by the JAK-STAT signaling pathway and through interferon regulatory factors (IRFs). IRFs are a family of transcription factors that possess a broad range of activities. IRF-3 is one of nine members which all share a common DNA binding domain which binds to an IFN stimulated response element (ISRE) found in the majority of IFN-inducible promoters. The IRF-3 gene expresses a 50 kDa protein which is constitutively expressed in all tissues. The protein undergoes post-translational modification as well as dimerization and is translocated from the cytoplasm to the nucleus upon viral infection or exposure to dsRNA. The antibody recognizes human IRF-3. A purified recombinant IRF-3 fusion protein corresponding to human IRF-3 (amino acids 56-427) was used as the immunogen.
Molecular Weight 50 kDa
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
Application Notes Clone SL-12.1 can be used for western blot analysis (0.5-2.0 µg/ml). Other reported applications include immunoprecipitation and immunofluorescence microscopy. The antibody will recognize both the nuclear and cytoplasmic forms of IRF-3 and can block IRF-3 DNA binding. Jurkat cells are recommended as a positive control (Cat, No. ABIN968537).
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-Interferon Regulatory Factor 3 (IRF3) (AA 56-427) antibody (ABIN967285) Western blot analysis of IRF-3. Lysates from Jurkat cells were probed with anti-human...
Product cited in: Hiscott, Pitha, Genin, Nguyen, Heylbroeck, Mamane, Algarte, Lin: "Triggering the interferon response: the role of IRF-3 transcription factor." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 19, Issue 1, pp. 1-13, 1999 (PubMed).

Mamane, Heylbroeck, Génin, Algarté, Servant, LePage, DeLuca, Kwon, Lin, Hiscott: "Interferon regulatory factors: the next generation." in: Gene, Vol. 237, Issue 1, pp. 1-14, 1999 (PubMed).

Ronco, Karpova, Vidal, Howley: "Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity." in: Genes & development, Vol. 12, Issue 13, pp. 2061-72, 1998 (PubMed).

Wathelet, Lin, Parekh, Ronco, Howley, Maniatis: "Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo." in: Molecular cell, Vol. 1, Issue 4, pp. 507-18, 1998 (PubMed).

Au, Moore, Lowther, Juang, Pitha: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, 1996 (PubMed).

Darnell, Kerr, Stark: "Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins." in: Science (New York, N.Y.), Vol. 264, Issue 5164, pp. 1415-21, 1994 (PubMed).

Loh, Chang, Fodor, Flavell: "Dissection of the interferon gamma-MHC class II signal transduction pathway reveals that type I and type II interferon systems share common signalling component(s)." in: The EMBO journal, Vol. 11, Issue 4, pp. 1351-63, 1992 (PubMed).

Reich, Evans, Levy, Fahey, Knight, Darnell: "Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 18, pp. 6394-8, 1987 (PubMed).