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BCL2 antibody (B-Cell CLL/lymphoma 2)

Details for Product anti-BCL2 Antibody No. ABIN967311, Supplier: Login to see New
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Synonyms AW986256, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik, PPP1R50, BCL-2, bcl-2, Bcl2
(680), (200), (150), (17), (9), (7), (4), (3), (2), (2), (1), (1)
(498), (242), (20), (6), (2), (2), (1)
Clonality (Clone)
Monoclonal ()
This BCL2 antibody is un-conjugated
(37), (33), (27), (24), (18), (14), (11), (10), (8), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(485), (275), (241), (144), (138), (135), (118), (68), (49), (42), (35), (11), (10), (10), (8), (8), (5), (1), (1), (1), (1), (1)
Pubmed 13 references available
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Quantity 50 μg
Shipping to United States ( )
Brand BD Pharmingen™
Immunogen Human Bcl-2 Recombinant Protein
Clone 6C8
Isotype IgG
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. The Bcl-2 gene was first found in t(14:18) containing follicular B-cell lymphomas. A high proportion of these lymphomas contains t(14:18) chromosomal translocations involving the human Bcl-2 gene. Translocation of Bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates Bcl-2 gene expression, resulting in high levels of Bcl-2 mRNA and protein expression. Because Bcl-2 blocks apoptosis it may contribute to tumorigenesis by prolonged cell survival rather than by accelerating the rate of cell proliferation. The reduced molecular weight of Bcl-2 is 26 kDa. Additional minor bands at 27-31 kDa and 18-21 kDa may also be observed.
Molecular Weight 26 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Related Products: ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store both the hamster anti-human Bcl-2 antibody and the Jurkat cell lysate undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (ABIN967311) Western blot analysis of Bcl-2. A Jurkat cell lysate (Human T-cell leukemia, ATCC TIB...
Background publications Martin, Veis, Korsmeyer et al.: "Latent membrane protein of Epstein-Barr virus induces cellular phenotypes independently of expression of Bcl-2." in: Journal of virology, Vol. 67, Issue 9, pp. 5269-78, 1993 (PubMed).

Veis, Sentman, Bach et al.: "Expression of the Bcl-2 protein in murine and human thymocytes and in peripheral T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2546-54, 1993 (PubMed).

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993 (PubMed).

Nguyen, Millar, Yong et al.: "Targeting of Bcl-2 to the mitochondrial outer membrane by a COOH-terminal signal anchor sequence." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25265-8, 1994 (PubMed).

Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, 1994 (PubMed).

Chiou, Rao, White: "Bcl-2 blocks p53-dependent apoptosis." in: Molecular and cellular biology, Vol. 14, Issue 4, pp. 2556-63, 1994 (PubMed).

Núñez, Merino, Grillot et al.: "Bcl-2 and Bcl-x: regulatory switches for lymphoid death and survival." in: Immunology today, Vol. 15, Issue 12, pp. 582-8, 1995 (PubMed).

Yang, Zha, Jockel et al.: "Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death." in: Cell, Vol. 80, Issue 2, pp. 285-91, 1995 (PubMed).

Batistatou, Merry, Korsmeyer et al.: "Bcl-2 affects survival but not neuronal differentiation of PC12 cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 10, pp. 4422-8, 1993 (PubMed).

Hockenbery, Oltvai, Yin et al.: "Bcl-2 functions in an antioxidant pathway to prevent apoptosis." in: Cell, Vol. 75, Issue 2, pp. 241-51, 1993 (PubMed).

Hockenbery, Nuñez, Milliman et al.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).

McDonnell, Nunez, Platt et al.: "Deregulated Bcl-2-immunoglobulin transgene expands a resting but responsive immunoglobulin M and D-expressing B-cell population." in: Molecular and cellular biology, Vol. 10, Issue 5, pp. 1901-7, 1990 (PubMed).

Hockenbery, Zutter, Hickey et al.: "BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 6961-5, 1991 (PubMed).

Catalog No. ABIN967311
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