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CD43 antibody (Sialophorin)

Details for Product anti-SPN Antibody No. ABIN967373, Supplier: Log in to see
Antigen
  • A630014B01Rik
  • CD43
  • Cd43
  • Galgp
  • GALGP
  • GPL115
  • gpL115
  • Lsn
  • LSN
  • Lsn1
  • Ly-48
  • Ly48
Reactivity
Mouse (Murine)
489
82
70
3
3
1
1
Host
Rat
454
110
77
Clonality (Clone)
Monoclonal ()
Conjugate
This CD43 antibody is un-conjugated
66
65
48
20
14
11
10
10
9
8
8
7
7
7
7
7
7
7
6
5
5
3
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
453
301
244
145
118
108
100
96
45
25
8
8
3
2
1
1
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Mouse Plasmacytoma MOPC-315
Clone S7
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD43 (Leukosialin) (SPN Antibody Abstract)
Background The S7 antibody reacts with the 115 kDa glycoform of CD43 (Ly-48, Leukosialin) which is expressed on IL-7-responsive pro-B cells, plasma cells, peritoneal and splenic CD5+ B cells (B-1 cells), granulocytes, monocytes, macrophages, platelets, natural killer cells, thymocytes, peripheral T cytotoxic/suppressor cells, and most T helper cells, but not resting conventional peripheral B cells. CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Ly-48, Leukosialin
Research Area CD Antigens
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Product cited in: Woodman, Johnston, Hickey, Teoh, Reinhardt, Poon, Kubes: "The functional paradox of CD43 in leukocyte recruitment: a study using CD43-deficient mice." in: The Journal of experimental medicine, Vol. 188, Issue 11, pp. 2181-6, 1999 (PubMed).

Thurman, Walker, Jayaraman, Manjunath, Ardman, Green: "Regulation of in vitro and in vivo T cell activation by CD43." in: International immunology, Vol. 10, Issue 5, pp. 691-701, 1998 (PubMed).

Stockton, Cheng, Manjunath, Ardman, von Andrian: "Negative regulation of T cell homing by CD43." in: Immunity, Vol. 8, Issue 3, pp. 373-81, 1998 (PubMed).

Rolink, ten Boekel, Melchers, Fearon, Krop, Andersson: "A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors." in: The Journal of experimental medicine, Vol. 183, Issue 1, pp. 187-94, 1996 (PubMed).

Manjunath, Correa, Ardman, Ardman: "Negative regulation of T-cell adhesion and activation by CD43." in: Nature, Vol. 377, Issue 6549, pp. 535-8, 1995 (PubMed).

Moore, Bennett, Kumar: "Transplantable NK cell progenitors in murine bone marrow." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 4, pp. 1653-63, 1995 (PubMed).

Sperling, Green, Mosley, Smith, DiPaolo, Klein, Bluestone, Thompson: "CD43 is a murine T cell costimulatory receptor that functions independently of CD28." in: The Journal of experimental medicine, Vol. 182, Issue 1, pp. 139-46, 1995 (PubMed).

Wells, Kantor, Stall: "CD43 (S7) expression identifies peripheral B cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 12, pp. 5503-15, 1995 (PubMed).

Jones, Federsppiel, Ellies, Williams, Burgener, Duronio, Smith, Takei, Ziltener: "Characterization of the activation-associated isoform of CD43 on murine T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3426-39, 1994 (PubMed).

Moore, Huang, Terstappen, Bennett, Kumar: "Expression of CD43 on murine and human pluripotent hematopoietic stem cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 4978-87, 1994 (PubMed).

Hardy, Hayakawa: "Generation of Ly-1 B cells from developmentally distinct precursors. Enrichment by stromal-cell culture or cell sorting." in: Annals of the New York Academy of Sciences, Vol. 651, pp. 99-111, 1992 (PubMed).

Hardy, Carmack, Shinton, Kemp, Hayakawa: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, 1991 (PubMed).

Gulley, Ogata, Thorson, Dailey, Kemp: "Identification of a murine pan-T cell antigen which is also expressed during the terminal phases of B cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 11, pp. 3751-7, 1988 (PubMed).