TER-119/Erythroid Cells antibody

Details for Product No. ABIN967375
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Antigen
Reactivity
Mouse (Murine)
Host
Rat
Clonality (Clone)
Monoclonal ()
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Cytotoxicity Test (CyTox), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)), Immunohistochemistry (Frozen Sections) (IHC (fro))
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967375
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Immunogen Mouse Fetal Liver
Clone TER-119
Isotype IgG2b, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background The TER-119 antibody reacts with a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the lineage cocktail used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Ly-76
Application Notes Immunohistochemistry: We recommend the use of the purified TER-119 antibody in our special formulation for IHC.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
General Ikuta, Kina, MacNeil et al.: "A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells." in: Cell, Vol. 62, Issue 5, pp. 863-74, 1990 (PubMed).

Maraskovsky, Brasel, Teepe et al.: "Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1953-62, 1996 (PubMed).

Kina, Ikuta, Takayama et al.: "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." in: British journal of haematology, Vol. 109, Issue 2, pp. 280-7, 2000 (PubMed).

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