Images for product: anti-Interferon gamma (IFNG) antibody
Expression of IFN-gamma by stimulated CD8+ and CD8-BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were cultured for 3 days in the presence of SEB (2 µg/ml, Sigma), then restimulated for 5 hr with hamster anti-mouse CD3 (2 µg/ml, 145-2C11) and hamster anti-mouse CD28 (2 µg/ml, 37.51) antibodies in the presence of 2 µg GolgiStop™ (aka Monensin). The splenocytes were harvested, stained with 0.06 µg of FITC-conjugated rat anti-mouse CD48 (FITC-53-6.7), fixed, permeabilized, and subsequently stained with 0.06 µg of PE-conjugated rat anti-mouse IFN-gamma antibody (PE-XMG1.2) by using Pharmingen's staining protocol (first panel). To demonstrate specificity of staining, the binding by the PE-XMG1.2 antibody was blocked by preincubation of the fixed/permeabilized cells with unlabeled XMG1.2 antibody (5.0 µg, second panel) prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.